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Environmental assessment of the building impact on the surroundings of Dukla barracks in Opava
Selník, Petr ; Kolínek, David
The carried out environmental study of the original area of the Dukel Barracks in Opava provides an assessment of the impact of buildings on its surroundings and establishes evaluation criteria for comparing the original solution of the area with the newly proposed solution. It is provided with an international evaluation methodology GREENPASS, which aims precisely at determining the functional parameters of construction projects, for example, also from the point of view of the new European agenda EU Taxonomy. The study evaluates the overheating of the surroundings, the possibility of creating a heat island, the effect of wind flow and ventilation, the degree of shading, changes in outflow parameters and CO2 sequestration. Dukla barracks in Opava is a set of historical military buildings from the end of the 19th century, which the Statutory City of Opava plans to revitalize and transform into residential buildings with an emphasis on the creation of a community space for the creation of social ties between its residents. The new design of the area is involved selected historical reconstructed barracks buildings, new green areas, sports fields, parking for residents and visitors. The area of warehouses, original stables and operational buildings will be replaced by new modern apartment buildings of a private developer. The presented study evaluates an area of 5 ha with a central courtyard, a sports field, original buildings and new apartment buildings.
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FISH method and its use in protistology
Frenclová, Martina ; Hampl, Vladimír (advisor) ; Zubáčová, Zuzana (referee)
Fluorescence in situ hybridization (FISH) is a technique that allows the localization and identification of specific sequences of nucleotides in DNA or RNA, which is subsequently visible under the microscope. FISH involves first denaturing the nucleic acids, either using high temperatures or by treatment with denaturing agents such as formamide.After subsequent induction of reasociation, the examined DNA or RNA pairs according to the complementarity rules with the short molecule called the probe, this process is called hybridization. Hybridization occurs in situ, that is within the examined specimen. Probes can be labeled either directly using fluorophores, or indirectly with a hapten, which is a substance having antigenic properties, which is subsequently detected using labeled antibodies or streptavidine. FISH has a large number of applications in molecular biology and medical science. In laboratory research in protistolgii FISH can be used for example to map the chromosomal genes to study the evolution of genome, analysis of nuclear organization or to confirmation of the origin of DNA sequence. Key words: FISH, fluorescence, probe, cell identification, environmental studies
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FISH method and its use in protistology
Frenclová, Martina ; Hampl, Vladimír (advisor) ; Zubáčová, Zuzana (referee)
Fluorescence in situ hybridization (FISH) is a technique that allows the localization and identification of specific sequences of nucleotides in DNA or RNA, which is subsequently visible under the microscope. FISH involves first denaturing the nucleic acids, either using high temperatures or by treatment with denaturing agents such as formamide.After subsequent induction of reasociation, the examined DNA or RNA pairs according to the complementarity rules with the short molecule called the probe, this process is called hybridization. Hybridization occurs in situ, that is within the examined specimen. Probes can be labeled either directly using fluorophores, or indirectly with a hapten, which is a substance having antigenic properties, which is subsequently detected using labeled antibodies or streptavidine. FISH has a large number of applications in molecular biology and medical science. In laboratory research in protistolgii FISH can be used for example to map the chromosomal genes to study the evolution of genome, analysis of nuclear organization or to confirmation of the origin of DNA sequence. Key words: FISH, fluorescence, probe, cell identification, environmental studies
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Comparison of ITS nrDNA and alternative markers for fungal metabarcoding in environmental samples
Zelenka, Tomáš ; Kolařík, Miroslav (advisor) ; Mašek, Tomáš (referee)
The study of fungal diversity may lead to many fundamental discoveries and conclusions. Molecular genetics, and particularly high throughput sequencing methods using short DNA fragments as barcodes, has recently experienced a boom. The most frequently used marker for fungal research is the partial region of nuclear ribosomal DNA called ITS (Internal Transcribed Spacer). It occurs in the form of tandem repetitions of up to 200 copies. This fact greatly simplifies its amplification from the environment but also introduces some negatives. One of them can be an existence of intragenomic and intraspecific variability which confounds diversity estimates by exaggerating the real number of species. Using alternative low-copy markers can easily prevent these problems. In this study EF-1α and RPB2 protein- coding genes were compared with traditionally used ITS1 and ITS2 markers. An artificial mock community was created by blending genomic DNA of different fungal lineages. The community was sequenced for all markers and the data were processed according to guidelines commonly used in environmental studies. The results show that ITS2 is unequivocally a more suitable marker for environmental studies than other compared markers. The average coefficient of overestimation was deemed to be approximately two for ITS1, ITS2,...
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